Energizing Cells from Within with NAD+ 1000mg

Nicotinamide adenine dinucleotide (NAD+) is growing in popularity for its potential involvement in age-related cellular changes, cellular communication and DNA repair.

You can find this coenzyme in every living cell. It’s involved in energy production and various essential metabolic processes.

Many people in longevity circles are exploring peptide therapy to help maintain and boost their ever-declining NAD+ levels. It happens naturally as you age and with that drop comes many of the symptoms of aging.

In this blog piece, we’ll detail what NAD+ is, how it supports mitochondrial health and influences the aging process. We’ll also explain the potential benefits that have made it such a popular research topic.

What is NAD+?

Nicotinamide adenine dinucleotide (NAD), or NAD+, the positively charged version, is a naturally occurring compound found in your cells. It plays a key role in hundreds of cell functions, such as cellular energy and helping regulate your body’s inflammatory and metabolic responses.

NAD+ isn’t only found in your body. It’s also active in plants, other mammals, bacteria and yeast.

Since its discovery in 1906, our understanding of how it works has grown. Scientists found that its precursor, niacin, identified in milk and yeast, was helpful with mitigating and alleviating the symptoms of a fatal disease called pellagra in the 1900s.

Scientists also realized that your NAD+ levels decline as you age, leading to the hypothesis that spurred its supplementation. They believed that a supplement version could help with the symptoms of metabolic disorders, muscle recovery and other age-related conditions.

This coenzyme plays a pivotal role in biological functions, leading to it becoming prized in the scientific research community. They’re all drawing connections between its benefits for animals and humans.

A focus of such research is how to maintain and increase NAD+ levels so people can reap the rewards of the potential benefits.

So, how does it produce them? This helper molecule is a coenzyme that binds to other enzymes. This characteristic allows it to cause molecular-level reactions.

How does NAD+ support mitochondrial health?

NAD+ indirectly activates mitochondrial function and biogenesis-regulating enzymes and participates directly with mitochondrial processes to support mitochondrial health.

This coenzyme is crucial for producing energy within mitochondria. It performs this function through metabolic pathways like the Tricarboxylic Acid (TCA) cycle and oxidative phosphorylation.

NAD+ also acts as a sirtuin substrate, supporting its activity and facilitating enzymatic reactions. These reactions influence cellular processes and gene expression, which have the potential to extend your lifespan and impact your health.

Sirtuins are enzymes that help maintain mitochondrial health by regulating processes like mitophagy (damaged mitochondria recycling) and mitochondrial biogenesis. They also manage functions like longevity, metabolism and stress responses.

NAD+ also plays a vital role in powering the body’s primary cellular energy source production, adenosine triphosphate (ATP). It does this by being a cofactor in the electron transport chain (ETC).

This peptide transports electrons between complexes as they flow through the ETC. This process enables ATP synthesis, which contributes to cellular energy metabolism.

How does NAD+ influence the aging process?

As the aging process transcends, levels of NAD+ decline, which causes a ripple effect of reduced DNA repair. It affects the regulation of energy metabolism and results in a less effective cellular stress response.

Reduced NAD+ levels may increase the chances of developing certain metabolic disorders like diabetes. It lowers the body’s ability to repair cells, raising inflammation and increasing aging markers like obesity and insulin resistance. 

Lower NAD+ levels can cause mitochondrial dysfunction, which is related to many conditions like muscular dystrophy and Alzheimer’s disease.

Mitochondrial dysfunction disrupts the electron transport chain, which lowers ATP synthesis, resulting in faulty cellular processes. It also influences the progression of age-related disorders like metabolic imbalances and neurodegeneration.

Studies show that interventions that increase NAD+ may slow aspects of the aging trajectory. Experiences with NAD+ replenishment in rodents have shown the following promising results: 

• Decrease chances of metabolic issues including alcoholic steatohepatitis and non-alcoholic steatohepatitis

• Reversal of glucose intolerance and mitochondrial dysfunction in younger mice 

• Protection of organs including protection of the liver against hepatotoxicity

• Improved capacity for organ recovery after hepatotoxicity and heart failure 

• Reduced arterial stiffness and increased endothelial functioning bring better cardiovascular health

• Improved cognition, neurological nerve protections and increased regeneration 

Why is NAD+ 1000mg popular?

NAD+ supplementation, specifically the 1000 mg dosage, is growing in popularity for its potential to support healthy aging and improve cardiovascular health and cognitive functioning.

Preclinical and clinical studies also suggest that precursors like nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) can help raise NAD+ levels.

Here are the top potential benefits leading to NAD+’s growing popularity:

NAD+ may slow some effects of aging

Researchers are studying NAD+ 1000 mg supplementation because of its potential to reduce, reverse or prevent age-related health conditions.

There’s a growing focus on human clinical trials observing how precursors like NR and NMN promote anti-aging effects. This research follows older animal studies that found NAD+ extended healthy aging and reduced premature aging signs.

NAD+ supplementation may improve cardiovascular health in middle-aged and older adults. It can do this by reducing the risk of arterial stiffness, high blood pressure and high cholesterol.

It may also offer anti-inflammatory effects and improve aging skeletal mass. These benefits could help prevent strength mass and muscle function loss over time.

NAD+ supplementation may also improve mitochondrial function in people with heart failure. It does this by enhancing peripheral blood mononuclear cell (PBMC) respiration and reducing proinflammatory cytokine gene expression.

NAD+ may reduce premature skin aging

Skin aging is one of the most recognizable signs of aging and it starts from the cellular level.

NAD+ can help repair DNA on a cellular level, which could slow the effects of aging and, in turn, could reduce the effects of aging on the skin.

It regulates cellular metabolism and may slow the aging trajectory, making it a potential preventative for premature skin aging. 

This peptide may also reduce inflammation and preserve muscle mass. It slows the aging of skeletal muscle, which can worsen the appearance of wrinkles. By maintaining skeletal muscle, it also reduces skin sagging, which commonly causes a more mature appearance. 

NAD+ may improve cognitive function

NAD+ supplementation could reduce nerve cell inflammation because of its potential link to anti-aging.

Nerve cell inflammation contributes to age-related cognitive disorders, including Alzheimer’s disease, Frontotemporal Dementia (FTD) and PArkinson’s disease.

A 2023 study shows evidence of a NAD+ deficiency in people living with Parkinson’s.

An earlier case report shows NAD+ treatment improving symptoms of Parkinson’s, including reducing vertical, horizontal and anterior-posterior axis tremors.

NAD+ supplementation may have a positive influence on Alzheimer’s disease-related dementia. Cross-species models show it improving cognitive deficits.

They may also protect against other neurodegenerative diseases like vascular dementia. It could also be a promising treatment strategy for traumatic brain injury, stroke and diabetes.

NAD+ may supports metabolic health

Metabolic health refers to the body’s ability to regulate insulin, blood sugar and cholesterol levels. NAD+ may aid in boosting metabolic health and reducing chances of metabolic disorders like diabetes. 

Studies show that NAD+ supplementation can increase insulin sensitivity and lipid levels, which helps stabilize blood sugar levels. By improving metabolic health, it can also reduce risk of conditions like stroke and heart disease. 

Safety and considerations for NAD+ research and use

NAD+ supplements haven’t shown any harmful side effects to humans unless exposed to excessive doses.

It’s important to note that NAD+ and its precursors are considered dietary supplements. The FDA doesn’t approve them for medical use yet.

NAD+ 1000mg is for research use only, to progress our understanding of what this peptide can do on a clinical level.

However, there are some potential drug interactions to be wary of before using this peptide:

• Anti-aging supplements: Supplements like quercetin or Q10 could boost NAD+ supplements’ effects because they’re also involved with the aging process.

• Antidepressants: some people may experience interference with their NAD levels when taking antidepressants. Taking them may reduce the effectiveness of NAD+ supplements.

• Insulin: People who require insulin medications may need different doses of NAD+ supplements to get the same effects. They can interact with your insulin sensitivity.

Always consult your healthcare provider if you take other supplements or prescribed medications. They can help you safely incorporate NAD+ supplements into your routine.

How to choose the right NAD+ supplement

Since NAD+ supplements aren’t FDA approved and are available at most health shops, finding pure and reliable forms of them is challenging.

Most of them are considered dietary supplements. So, you can’t focus solely on a popular brand—you need to consider whether it’s effective, clean and right for you.

Here’s what you should consider:

• Purity: An ideal NAD+ supplement clearly displays its active ingredient’s purity level, for example, “purity exceeds 99%”.

• Testing: Look for companies that offer rigorous quality control and testing processes to ensure the purity of their products.

• Dosage routine: Consider the per serving dosage and how often you need to take a serving.

• Admission route: Consider what route of admission suits you best—sublingual tablets, powders, capsules or injectable liquids. While powders, sublinguals and capsules are more convenient, injections are typically more effective.

• Cost vs. quality: Generic blends may be cheaper than pure NAD+ supplements but aren’t as effective or transparent.

Ethical guidelines for NAD+ research

When conducting NAD+ research, it’s essential to adhere to general scientific principles of integrity, responsible conduct and participant protection.

Considering the potential benefits and risks of NAD+ interventions in human studies are especially critical.

Here’s how you to practice ethical research:

• Consider how different levels of NAD+ could influence the outcomes of interventions.

• Protect your participants’ privacy by adhering to institutional data sharing and storage regulations.

• Get informed consent by ensuring participants understand the risks, benefits and purpose of its supplementation.

• Be accountable for research activities and transparent about findings and research methods.

• Adhere to local approved protocols and ethical guidelines for animal euthanasia and care when conducting animal studies.

• Evaluate NAD+’s potential risks carefully. Consider serious adverse effects on circadian rhythm, inflammation and feedback inhibition.

Lab protocols for NAD+ research

NAD+ research should prioritize proper sample handling, accurate quantification and validated level detection methods.

Here are some recommended lab protocols to follow:

• Use validated methods like enzymatic cycling assays and liquid chromatography tandem mass spectrometry (LC-MS/MS) for NAD+ quantification.

• Consider potential statistical significance and confounding factors by thoroughly analyzing your data.

• Ensure accuracy and reproducibility by using standard protocols and solutions for NAD+ quantification. Other researchers should also be able to replicate these protocols.

• Use appropriate standards and controls to validate NAD+ quantification and detection methods.

• Ensure accurate measurements by correctly storing, collecting and processing samples. Consider how pre-analytical factors, like temperature and storage time, can affect NAD+ concentrations.

• Follow established sample preparation protocols like extraction of NAD+ and lysis of tissues and cells.

Empower your cells from within with Spartan Peptides

NAD+ is deeply involved in mitochondrial processes, supporting mitochondrial health. Interventions that increase it may slow elements of the aging trajectory.

Other potential benefits like improved cognitive function, decreased premature skin aging and metabolic health support have grown the popularity of NAD+ supplementation and its research.

NAD+ is generally considered safe for human use.

When conducting research it’s essential to follow ethical guidelines and lab protocols. Doing this also improves the accuracy and reliability of your findings and the replicability and reproducibility of your methods.

To choose the right NAD+ supplement, focus on purity, testing and quality over cost. Head to Spartan Peptides to find NAD+ 1000mg with over 99% purity.