HPLC
High-Performance Liquid Chromatography, an analytical separation technique used to verify the purity and identity of research peptide compounds.
Definition
High-Performance Liquid Chromatography (HPLC) is an analytical technique that separates, identifies, and quantifies components of a mixture by passing it through a column packed with stationary phase material under high pressure with a flowing liquid mobile phase. Different components travel through the column at different rates depending on their physical and chemical interactions with the stationary phase, allowing separation and detection. For research peptides, reversed-phase HPLC (RP-HPLC) is the standard method for purity analysis, using a hydrophobic stationary phase and an aqueous-organic mobile phase gradient. Purity is expressed as the percentage of the main peak area relative to total integrated peak area.
Research Context
HPLC purity analysis is the primary quality assurance method for research-grade peptide compounds. Spartan Peptides provides compounds meeting at least 98% HPLC-verified purity, established by RP-HPLC analysis with UV detection at 214 or 220 nm (peptide bond absorption wavelength). HPLC purity data appears on the certificate of analysis for each lot and is the industry standard for verifying that a research peptide compound contains the claimed active component at the specified purity level. Researchers selecting peptide compounds for rigorous preclinical studies should always request and review HPLC data before use.
Relevant Compounds
This term applies to the following research compound hubs.
Frequently Asked Questions
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